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Part:BBa_M1531

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-04-18)


Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

This is a thermal hysteresis (antifreeze) protein with an 8-His tag for protein purification. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL1. There is a secretion tag, and a portion of the sequence causes the cell to secrete GFP as well to measure the activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4788
    Illegal SpeI site found at 943
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2261
    Illegal BglII site found at 2924
    Illegal BglII site found at 6818
    Illegal BglII site found at 7481
    Illegal BamHI site found at 5307
    Illegal XhoI site found at 5208
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 943
    Illegal NgoMIV site found at 1191
    Illegal NgoMIV site found at 5748
    Illegal AgeI site found at 678
    Illegal AgeI site found at 4430
    Illegal AgeI site found at 4487
    Illegal AgeI site found at 8987
    Illegal AgeI site found at 9044
  • 1000
    COMPATIBLE WITH RFC[1000]


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